“Somos microscópicos en la inmensidad del universo, como las bacterias lo son para nosotros” señaló María Laura Echarren, miembro del @ibr_rosario (IBR, CONICET-UNR) en relación a su trabajo “Una Odisea de Salmonelosis”, que recibió el premio People’s Choice de la Sociedad Americana de Microbiología.
Más información del trabajo de Echarren, en esta nota: https://bit.ly/2GK6T1D
Lysine Iron Agar is used to distinguish between bacterial pathogens, specifically intestinal pathogens in the Enterobacteriaceae family. This agar is usually a slant in a tube and after inoculation and incubation scientists are able to determine if lysine has been decarboxylated or deaminated by the microorganism, helping them to identify the organism. This slant is inoculated with a needle, and a small amount of a bacterial colony picked up on a sterilized needle is then stabbed to the bottom of the tube (commonly referred to as the butt🍑), and the slant is streaked with the same needle as it is being withdrawn. It is then incubated in 35°C air (with loose lids!) for 18-48 hours before the reaction is read.
The color of the butt indicates whether lysine decarboxylation has occurred, with purple 💜 (alkaline) indicating a positive result and yellow 💛 (acid) indicating negative. Lysine deamination is detected on the slant, with a red ❤️ slant indicating a positive result and purple indicating a negative result. This agar can also tell scientists if a MO produces H2S. If the butt of the tube is black 🖤 the bacteria produces H2S, which is very helpful when identifying Salmonella, an H2S producer that can be highly pathogenic.
In most labs, these reactions are abbreviated as seen in the photo. The purple indicates an alkaline pH and is abbreviated “K,” while the yellow indicates an acid pH and is abbreviated “A;” the red is abbreviated “R.” So for example, the E. coli is K/K, meaning it is positive for lysine decarboxylation but negative for lysine deamination. These biochemical tests are super cool and also super pretty 😍. #labsciences#laboratory#medicallaboratoryscience#medicallaboratoryscientist#lab#labtesting#diagnostictesting#science#clinicallabscience#clinicallab#microscope#microscopic#ladiesinlabcoats#womeninstem#womeninscience#microbiology#bacteria#salmonella#shigella#bugs#bugfarmer#microbes#microorganisms
Things got interesting when Messy Missy made an appearance in lab yesterday and made a bottle of fushia dye explode. ✓ Notebooks
✓Floor, table, chairs
✓ Fellow Students Faces
✓Lab coats + My sweater sleeve
✓Very expensive Microscope
Still finding drops of fushia all over myself.
But my slide stain turned out perfect!